糖原合酶激酶3β(GSK3b)重組蛋白
Recombinant Glycogen Synthase Kinase 3 Beta (GSK3b)
Serine/threonine-protein kinase GSK3B
- 編號RPD317Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細(xì)胞定位細(xì)胞膜, 細(xì)胞核, 細(xì)胞質(zhì)
- 預(yù)測分子量32.6kDa
- 實際分子量33kDa(差異分析請參閱說明書)
- 片段與標(biāo)簽Lys122~Thr375 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 97%
- 等電點7.8
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPD317Ra01 | 糖原合酶激酶3β(GSK3b)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAD317Ra01 | 糖原合酶激酶3β(GSK3b)多克隆抗體 | WB; IHC; ICC; IP. |
MAD317Ra21 | 糖原合酶激酶3β(GSK3b)單克隆抗體 | WB; IHC; ICC; IP. |
SED317Ra | 糖原合酶激酶3β(GSK3b)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMD317Ra | 糖原合酶激酶3β(GSK3b)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Neurological Research | Sorafenib and lithium chloride combination treatment shows promising synergistic effects in human glioblastoma multiforme cells in vitro but midkine is not implicated [Maneyonline:Source] |
?Int J Clin Exp Med | Glycogen synthase kinase-3β (GSK-3β) rs334558 polymorphism is not associated with mild cognitive impairment in Chinese Han type 2 diabetic patients [ijcem0038535.pdf] |
Bulletin?of Experimental?Biology?and?Medicine | Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia [Pubmed:29308565] |
Biomedicine & Pharmacotherapy | Neuroprotective effects of Bergenia ciliata on NMDA induced injury in SH-SY5Y cells and attenuation of cognitive deficits in scopolamine induced amnesia in rats [Pubmed: 30227331] |
FEATURES OF DISTRIBUTION OF SOYBEAN AGGLUTININ (SBA) RECEPTORS IN THE EXTRACELLULAR MATRIX OF THE MENISCI OF RAT KNEE JOINT?… [] | |
Br J Haematol | Glycogen synthase kinase 3β/CCR6‐positive bone marrow cells correlate with disease activity in multicentric Castleman disease‐TAFRO [34873687] |
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