腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)
High Sensitive ELISA Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2)
FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein
- 編號HEA559Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測范圍78-5,000pg/mL
- 靈敏度最小可檢測劑量小于等于34pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 2302 ¥ 3288 ¥ 14796 ¥ 27948 ¥ 230160
- 欲了解實(shí)際交易價(jià)格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實(shí)驗(yàn)結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 94-101 | 97 |
| EDTA plasma(n=5) | 97-105 | 102 |
| heparin plasma(n=5) | 87-94 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 80-101% | 78-104% | 79-90% |
| EDTA plasma(n=5) | 90-102% | 93-101% | 78-95% | 81-94% |
| heparin plasma(n=5) | 82-103% | 78-104% | 78-88% | 81-93% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPA559Hu61 | 腸型脂肪酸結(jié)合蛋白(FABP2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA559Hu01 | 腸型脂肪酸結(jié)合蛋白(FABP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA559Hu01 | 腸型脂肪酸結(jié)合蛋白(FABP2)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA559Hu22 | 腸型脂肪酸結(jié)合蛋白(FABP2)單克隆抗體 | WB; IHC; ICC; IP. |
| HEA559Hu | 腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA559Hu | 腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA559Hu | 腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Food Chem | Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes [PubMed: 25952883] |
| Oxid Med Cell Longev | Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation Model [PubMed: 26064429] |
| Vet Immunol Immunopathol | Immunomodulatory potential of β-glucan as supportive treatment in porcine rotavirus enteritis. [pubmed:28895864] |
| PLoS One | Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats [pubmed:28622357] |
| Journal of Immunology and Immunopathology | Effect of Porcine Rotavirus Enteritis on Concentrations of Interferonγ, Immunoglobulin-G and Intestinal Fatty Acid-Binding Protein-2 and Peripheral Leukocyte Function [10.5958/0973-9149.2017.00011.9] |
| Cellular Physiology and Biochemistry | Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory?… [Pubmed:29672286] |
| European?Journal of Clinical Nutrition | Lack of liver steatosis in germ-free mice following hypercaloric diets [Pubmed:29926176] |
| Nutrients | Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron [Pubmed:29861488] |
| International?Surgery?Journal | Intestinal fatty acid binding protein (I-FABP) as a marker for acute intestinal ischemia [Doi: 10.18203/2349-2902.isj20190058] |
| Hepatology?Communications | Effect of a Multistrain Probiotic on Cognitive Function and Risk of Falls in Patients With Cirrhosis: A Randomized Trial [] |
| Tropical?Animal?Health?and?Production | Immunomodulating dose of levamisole stimulates innate immune response and prevents intestinal damage in porcine rotavirus diarrhea: a restricted-randomized?… [Pubmed: 30790158] |
| Biological & Pharmaceutical Bulletin | Role of Aquaporin-3 in Intestinal Injury Induced by Sepsis [Pubmed: 31582652] |
| International Journal of Medical Sciences | FABP1 and FABP2 as markers of diabetic nephropathy [Pubmed: 32922200] |
| Int Immunopharmacol | Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory response [Pubmed: 32361189] |
| Journal of Exercise Physiology Online | Markers of Intestinal Damage in Individuals with and without Obesity during a 12-Week Exercise Period. [] |
| The Journal of Nutritional Biochemistry | Effects of fucoidan on tumor prevention and gut flora [] |
| european journal of clinical nutrition? | Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury [33514869] |
| J Cell Mol Med | The |á2AR/Caveolin?\1/p38MAPK/NF?\|êB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia?\reperfusion [34114328] |
| BMC Gastroenterol | Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity [34022802] |
| JOURNAL OF APPLIED PHYSIOLOGY | Impact of Successive Exertional Heat Injuries on Thermoregulatory and Systemic Inflammatory Responses in Mice [34528459] |
| PLoS One | The protective effects of HIF-1α activation on sepsis induced intestinal mucosal barrier injury in rats model of sepsis [Pubmed:35576220] |
