白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Interleukin 1 Receptor Antagonist (IL1RA) ,etc. by FLIA (Flow Luminescence Immunoassay)
IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra
(注:單次混測多因子不超過8個指標 )
- 編號LMA223Hu
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍1.95-2000pg/mL
- 靈敏度最小可檢測劑量小于等于0.65 pg/mL.
- 樣本類型Serum, plasma, tissue homogenatesand other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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特異性
本試劑盒用于檢測白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 99-105 | 102 |
EDTA plasma(n=5) | 94-102 | 97 |
heparin plasma(n=5) | 82-103 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 93-104% | 91-98% | 83-101% | 93-101% |
EDTA plasma(n=5) | 90-99% | 80-98% | 78-98% | 99-105% |
heparin plasma(n=5) | 96-103% | 88-96% | 98-105% | 95-104% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠)標準品或樣本100μL及磁珠10μL,
37°C酶標板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測溶液A100μL,37°C酶標板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠,其中的白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體,將未結(jié)合的生物素化抗體洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈品
增值服務
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
APA223Hu01 | 白介素1受體拮抗劑(IL1RA)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA223Hu01 | 白介素1受體拮抗劑(IL1RA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA223Hu61 | 白介素1受體拮抗劑(IL1RA)活性蛋白 | Cell?culture;?Activity?Assays. |
EPA223Hu61 | 白介素1受體拮抗劑(IL1RA)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA223Hu01 | 白介素1受體拮抗劑(IL1RA)多克隆抗體 | WB; IHC; ICC; IP. |
PAA223Hu06 | 白介素1受體拮抗劑(IL1RA)多克隆抗體 | WB; IHC; ICC; IP. |
FAA223Hu01 | 抗白介素1受體拮抗劑(IL1RA)多克隆抗體 | Flow cytometry. |
LAA223Hu71 | 白介素1受體拮抗劑(IL1RA)多克隆抗體(生物素標記) | WB; IHC; ICC. |
LAA223Hu81 | 白介素1受體拮抗劑(IL1RA)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
MAA223Hu26 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
MAA223Hu22 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
MAA223Hu24 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
MAA223Hu25 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
MAA223Hu21 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
MAA223Hu27 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
MAA223Hu23 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
LAA223Hu72 | 白介素1受體拮抗劑(IL1RA)單克隆抗體(生物素標記) | WB; IHC; ICC. |
HEA223Hu | 白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEA223Hu | 白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA223Hu | 白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSA223Hu01 | 白介素1受體拮抗劑(IL1RA)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA223Hu01 | 白介素1受體拮抗劑(IL1RA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
The Veterinary Journal | Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease [ScienceDirect: S1090023312000962] |
Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horses [Researchgate:Source] | |
Journal of Equine Veterinary Science | Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1 [Science: Article] |
Journal of Equine Veterinary Science | A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol Concentrations [Science: Article] |
Scientific publications in peer reviewed journals | Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horse [receive:101658] |
Multiple Sclerosis and Related Disorders | Interleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrain [pubmed:29141788] |
Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267] |
Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533] |
Journal of Veterinary?Internal?Medicine | Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease [Pubmed:29572935] |
Frontiers in Pharmacology | M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice [Pubmed: 33041798] |