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白介素1受體拮抗劑(IL1RA)活性蛋白

Active Interleukin 1 Receptor Antagonist (IL1RA)

IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra

  • 白介素1受體拮抗劑(IL1RA)活性蛋白 產(chǎn)品包裝(模擬)
  • 白介素1受體拮抗劑(IL1RA)活性蛋白 產(chǎn)品包裝(模擬)
  • 白介素1受體拮抗劑(IL1RA)活性蛋白 Figure. Gene Sequencing (extract)
  • APA223Hu61.png Figure. SDS-PAGE
  • 白介素1受體拮抗劑(IL1RA)活性蛋白 Sample: Recombinant IL1RA, Human;
    Antibody: Rabbit Anti-Human IL1RA Ab (PAA223Hu06)
    Figure. Western Blot
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

IL-1RA is an agent that binds non-productively to the cell surface?interleukin-1 receptor?(IL-1R), the same receptor that binds?interleukin 1?(IL-1), preventing IL-1 from sending a signal to that cell. Besides, Interleukin 1 Alpha (IL-1a) has been identified as an interactor of IL-1RA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL-1RA and recombinant human IL-1a. Briefly, IL1RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to IL-1a-coated microtiter wells and incubated for 2 h at 37℃. Wells were washed with PBST and incubated for 1 h with anti-IL-1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL-1RA and IL-1a was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of IL-1RA with IL-1a.

用法

Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
APA223Hu01 白介素1受體拮抗劑(IL1RA)活性蛋白 Cell?culture;?Activity?Assays.
RPA223Hu01 白介素1受體拮抗劑(IL1RA)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA223Hu61 白介素1受體拮抗劑(IL1RA)活性蛋白 Cell?culture;?Activity?Assays.
EPA223Hu61 白介素1受體拮抗劑(IL1RA)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA223Hu01 白介素1受體拮抗劑(IL1RA)多克隆抗體 WB; IHC; ICC; IP.
PAA223Hu06 白介素1受體拮抗劑(IL1RA)多克隆抗體 WB; IHC; ICC; IP.
FAA223Hu01 抗白介素1受體拮抗劑(IL1RA)多克隆抗體 Flow cytometry.
LAA223Hu71 白介素1受體拮抗劑(IL1RA)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
LAA223Hu81 白介素1受體拮抗劑(IL1RA)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
MAA223Hu26 白介素1受體拮抗劑(IL1RA)單克隆抗體 WB; IHC; ICC; IP.
MAA223Hu22 白介素1受體拮抗劑(IL1RA)單克隆抗體 WB; IHC; ICC; IP.
MAA223Hu24 白介素1受體拮抗劑(IL1RA)單克隆抗體 WB; IHC; ICC; IP.
MAA223Hu25 白介素1受體拮抗劑(IL1RA)單克隆抗體 WB; IHC; ICC; IP.
MAA223Hu21 白介素1受體拮抗劑(IL1RA)單克隆抗體 WB; IHC; ICC; IP.
MAA223Hu27 白介素1受體拮抗劑(IL1RA)單克隆抗體 WB; IHC; ICC; IP.
MAA223Hu23 白介素1受體拮抗劑(IL1RA)單克隆抗體 WB; IHC; ICC; IP.
LAA223Hu72 白介素1受體拮抗劑(IL1RA)單克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
HEA223Hu 白介素1受體拮抗劑(IL1RA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA223Hu 白介素1受體拮抗劑(IL1RA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA223Hu 白介素1受體拮抗劑(IL1RA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
PSA223Hu01 白介素1受體拮抗劑(IL1RA)抗體對(duì) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA223Hu01 白介素1受體拮抗劑(IL1RA)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志 參考文獻(xiàn)
The Veterinary Journal Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease [ScienceDirect: S1090023312000962]
Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horses [Researchgate:Source]
Journal of Equine Veterinary Science Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1 [Science: Article]
Journal of Equine Veterinary Science A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol Concentrations [Science: Article]
Scientific publications in peer reviewed journals Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horse [receive:101658]
Multiple Sclerosis and Related Disorders Interleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrain [pubmed:29141788]
Advances in Medical Sciences A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267]
Archives of Medical Research Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533]
Journal of Veterinary?Internal?Medicine Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease [Pubmed:29572935]
Frontiers in Pharmacology M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice [Pubmed: 33041798]
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