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神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)

Mini Samples ELISA Kit for Neuropeptide Y (NPY)

NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

  • 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) 產(chǎn)品包裝(模擬)
  • 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) 產(chǎn)品包裝(模擬)
  • 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) 實(shí)驗(yàn)結(jié)果圖
  • MEA879Mu.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 94-102 98
EDTA plasma(n=5) 95-102 98
heparin plasma(n=5) 90-103 97

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 89-104% 78-95% 82-99%
EDTA plasma(n=5) 92-103% 78-98% 91-99% 91-99%
heparin plasma(n=5) 80-102% 96-104% 95-105% 98-105%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）25µL后，立即加入檢測溶液A25µL，
    37°C孵育1小時(shí)；
3. 甩干，洗板3次；
4. 加檢測溶液B50µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分鐘；
7. 加終止液25µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA879Mu02 神經(jīng)肽Y(NPY)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA879Mu01 神經(jīng)肽Y(NPY)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA879Mu21 神經(jīng)肽Y(NPY)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
PAA879Mu02 神經(jīng)肽Y(NPY)多克隆抗體 WB; IHC; ICC; IP.
PAA879Mu01 神經(jīng)肽Y(NPY)多克隆抗體 WB; IHC; ICC; IP.
MEA879Mu 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA879Mu 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA879Mu 神經(jīng)肽Y(NPY)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA879Mu 神經(jīng)肽Y(NPY)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA879Mu11 神經(jīng)肽Y(NPY)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Hemodialysis International Is there any interaction of resistin and adiponectin levels with protein‐energy wasting among patients with chronic kidney disease [Onlinelibrary: hdi.12072]
Journal of Pediatric Endocrinology and Metabolism Changes in growth pattern, leptin ghrelin and neuropeptide Y levels after adenotonsillectomy in prepubertal children [Degruyter: Source]
Ren Fail. The role of adipocytokines on depressive symptoms of patients with chronic kidney disease [Pubmed: 23879728]
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development. [Pubmed: 24077326]
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development [Lww:Source]
Toxicology Letters Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate [Pubmed:25623392]
J Neurosurg Anesthesiol High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid Hemorrhage [PubMed: 25955865]
Journal of Diabetes Research High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate … [Pubmed:26885531]
Archives of Gynecology and Obstetrics Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetate [pubmed:27604241]
basic & clinical pharmacology & toxicology Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells. [pubmed:27625336]
Acta?Neurobiologiae Experimentalis Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat [pubmed:27685769]
Cardiovascular?research Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axis [Pubmed:29850786]
Journal of Periodontology Salivary neuropeptides, stress, and periodontitis [Pubmed:28914594]
Neuropeptides Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor [Pubmed:29685637]
Pharmacology Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y?… [Pubmed: 30227410]
Marmara Medical Journal Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome []
Marmara Medical Journal Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome. []
neurochemical research Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats. [Pubmed: 32378074]
Hum Psychopharmacol Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double?\blind randomized??- [33245168]
Arch Toxicol Serotonin involvement in okadaic acid-induced diarrhoea in vivo [34148100]
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