心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)
Mini Samples ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)
CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T
- 編號MED232Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.0pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3528 ¥ 5040 ¥ 22680 ¥ 42840 ¥ 352800
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-93 | 89 |
| EDTA plasma(n=5) | 80-104 | 95 |
| heparin plasma(n=5) | 88-98 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-105% | 85-96% | 85-102% | 83-97% |
| EDTA plasma(n=5) | 80-96% | 90-98% | 83-94% | 98-105% |
| heparin plasma(n=5) | 98-105% | 82-99% | 79-99% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）25µL，37°C溫育1小時；
3. 吸棄，加檢測溶液A25µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B25µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分鐘；
8. 加終止液20µL，立即450nm讀數(shù)。
實驗原理
將心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)與連接于固相載體上的抗體結合，然后加入生物素化的心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPD232Hu01 | 心肌肌鈣蛋白T(TNNT2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD232Hu03 | 心肌肌鈣蛋白T(TNNT2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD232Hu04 | 心肌肌鈣蛋白T(TNNT2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD232Hu02 | 心肌肌鈣蛋白T(TNNT2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD232Hu01 | 心肌肌鈣蛋白T(TNNT2)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAD232Hu03 | 心肌肌鈣蛋白T(TNNT2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAD232Hu01 | 心肌肌鈣蛋白T(TNNT2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAD232Hu02 | 心肌肌鈣蛋白T(TNNT2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAD232Hu81 | 心肌肌鈣蛋白T(TNNT2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAD232Hu71 | 心肌肌鈣蛋白T(TNNT2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAD232Hu23 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu29 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu24 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu25 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu21 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu22 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu27 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu28 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| FAD232Hu08 | 抗心肌肌鈣蛋白T(TNNT2)單克隆抗體 | FCM |
| FAD232Hu02 | 抗心肌肌鈣蛋白T(TNNT2)單克隆抗體 | Flow cytometry. |
| FAD232Hu04 | 抗心肌肌鈣蛋白T(TNNT2)單克隆抗體 | Flow cytometry. |
| FAD232Hu06 | 抗心肌肌鈣蛋白T(TNNT2)單克隆抗體 | Flow cytometry. |
| SED232Hu | 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HED232Hu | 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MED232Hu | 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCD232Hu | 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMD232Hu | 心肌肌鈣蛋白T(TNNT2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSD232Hu01 | 心肌肌鈣蛋白T(TNNT2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Basic & Clinical Pharmacology & Toxicology | Cardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in Rats [PubMed: 21232020] |
| Basic & Clinical Pharmacology & Toxicology | Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats [Wiley: source] |
| European Journal of Pharmacology | Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats [ScienceDirect: S0014299909009984] |
| Journal of translational medicine | The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury [PubMed: PMC3694448] |
| Oral Diseases | Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction [Pubmed: 22834943] |
| An International Journal of Pharmaceutical Sciences | In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium [Pubmed: 23700796] |
| Journal of Animal and Feed Sciences, | The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats [Pl:Source] |
| Liver Int. | Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosis [Pubmed:24330504] |
| Cardiovasc Toxicol. | Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in Pigs [Pubmed:24420420] |
| British Journal of Pharmacology | Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise [Pubmed:25039894] |
| Chemico-Biological Interactions | Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways [Pubmed:26807765] |
| The Journal of Pharmacology and Experimental Therapeuties | The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice [Pubmed:27247000] |
| The Journal of Maternal-Fetal & Neonatal Medicine | Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies. [pubmed:27937003] |
| PLoS One. | Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. [pubmed:27907155] |
| Biomedicine & Pharmacotherapy | Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats [pubmed:28076830] |
| PLoS One | Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model [pubmed:28570599] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus [pubmed:29042932] |
| PLoS One | Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury [Pubmed: 30192780] |
| PLoS One | Tissue suction-mediated gene transfer to the beating heart in mice [Pubmed: 32027678] |
| Journal of Human Kinetics | Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy [Pubmed: 32148580] |
| BIOMEDICINE & PHARMACOTHERAPY | Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress [Pubmed: 33022535] |
| Toxics | Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in Rats [Pubmed: 32947820] |
| Elife | Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice [33526170] |
| Cell Metabolism | Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species [34536344] |
| Anal Bioanal Chem | Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications [34673993] |
| Experimental and Therapeutic Medicine | Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest [Pubmed:35495584] |
