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70kDa熱休克蛋白5(HSPA5)單克隆抗體

Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)

MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78

編號MAC343Mu23
物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(克隆號)C11
Ig亞型 IgG2a Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPC343Mu01-70kDa熱休克蛋白5(HSPA5)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/mL
且適物種Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠)
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
價格 ¥ 711 1659 ¥ 1659 ¥ 2370 ¥ 5925 ¥ 23700
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Mouse Testis Tissue; Primary Ab: 20μg/ml Mouse Anti-Mouse HSPA5 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 20μg/ml Mouse Anti-Mouse HSPA5 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue
Primary Ab: 20μg/ml Mouse Anti-Mouse HSPA5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Mouse Testis Tissue
Primary Ab: 20μg/ml Mouse Anti-Mouse HSPA5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Liver lysate; Lane3: Rat Testis lysate; Lane4: A549 cell lysate Primary Ab: 2μg/ml Mouse Anti-Mouse HSPA5 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對HSPA5的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗中能識別HSPA5。

用法

Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗服務(wù)
免疫沉淀(IP)實(shí)驗服務(wù)
免疫熒光(IF)實(shí)驗服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時熒光定量PCR實(shí)驗服務(wù)

相關(guān)產(chǎn)品

編號	適用物種：Mus musculus (Mouse，小鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPC343Mu02	70kDa熱休克蛋白5(HSPA5)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPC343Mu01	70kDa熱休克蛋白5(HSPA5)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
EPC343Mu61	70kDa熱休克蛋白5(HSPA5)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPC343Mu03	70kDa熱休克蛋白5(HSPA5)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAC343Mu02	70kDa熱休克蛋白5(HSPA5)多克隆抗體	WB; IHC; ICC; IP.
PAC343Mu01	70kDa熱休克蛋白5(HSPA5)多克隆抗體	WB; IHC; ICC; IP.
LAC343Mu71	70kDa熱休克蛋白5(HSPA5)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAC343Mu25	70kDa熱休克蛋白5(HSPA5)單克隆抗體	WB; IHC; ICC; IP.
MAC343Mu22	70kDa熱休克蛋白5(HSPA5)單克隆抗體	WB; IHC; ICC; IP.
MAC343Mu21	70kDa熱休克蛋白5(HSPA5)單克隆抗體	WB; IHC; ICC; IP.
MAC343Mu27	70kDa熱休克蛋白5(HSPA5)單克隆抗體	WB; IHC; ICC; IP.
MAC343Mu23	70kDa熱休克蛋白5(HSPA5)單克隆抗體	WB; IHC; ICC; IP.
MAC343Mu24	70kDa熱休克蛋白5(HSPA5)單克隆抗體	WB; IHC; ICC; IP.
SEC343Mu	70kDa熱休克蛋白5(HSPA5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
SCC343Mu	70kDa熱休克蛋白5(HSPA5)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
LMC343Mu	70kDa熱休克蛋白5(HSPA5)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSC343Mu01	70kDa熱休克蛋白5(HSPA5)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)	Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志	參考文獻(xiàn)
41	Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. [PubMed: 20522592]
pharmacological research	Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors [Pubmed: 31048034]
EPILEPSY & BEHAVIOR	Fenfluramine acts as a positive modulator of sigma-1 receptors [Pubmed: 32169824]
Journal of Oncology	Using Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer [33381181]
Heliyon	Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior []
Medicine (Baltimore)	Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients [34414940]

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