嵌套蛋白(NES)單克隆抗體
Monoclonal Antibody to Nestin (NES)
- 編號MAA500Hu24
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C1
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPA500Hu01-嵌套蛋白(NES)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 625 ¥ 1457 ¥ 2082 ¥ 5205 ¥ 20820
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 30μg/ml Mouse Anti-Human NES Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Glioma Tissue;
Primary Ab: 30μg/ml Mouse Anti-Human NES Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
AF488 staining on IF;
Sample: SH-SY5Y cell
Primary Ab: 20μg/ml Mouse Anti-Human NES Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Human SH-SY5Y cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human NES Monoclonal Antibody (Catalog MAA500Hu24, red histogram) or Isotype control antibody (Catalog IS067-Mu02, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對NES的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別NES。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA500Hu01 | 嵌套蛋白(NES)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA500Hu01 | 嵌套蛋白(NES)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA500Hu51 | 嵌套蛋白(NES)多克隆抗體(別藻藍蛋白標記) | WB; IHC; ICC; IF. |
| MAA500Hu27 | 嵌套蛋白(NES)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA500Hu21 | 嵌套蛋白(NES)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA500Hu29 | 嵌套蛋白(NES)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA500Hu23 | 嵌套蛋白(NES)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA500Hu24 | 嵌套蛋白(NES)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA500Hu25 | 嵌套蛋白(NES)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA500Hu22 | 嵌套蛋白(NES)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA500Hu26 | 嵌套蛋白(NES)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA500Hu28 | 嵌套蛋白(NES)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA500Hu02 | 抗嵌套蛋白(NES)單克隆抗體 | FCM |
| FAA500Hu08 | 抗嵌套蛋白(NES)單克隆抗體 | Flow cytometry. |
| FAA500Hu04 | 抗嵌套蛋白(NES)單克隆抗體 | Flow cytometry. |
| FAA500Hu06 | 抗嵌套蛋白(NES)單克隆抗體 | Flow cytometry. |
| SEA500Hu | 嵌套蛋白(NES)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA500Hu | 嵌套蛋白(NES)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Kidney?and?Blood?Pressure?Research | Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5 [Pubmed:29689548] |
| Neural Plast | P2Y2 Receptor Mediated Neuronal Regeneration and Angiogenesis to Affect Functional Recovery in Rats with Spinal Cord Injury [Pubmed:35154311] |
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