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抗增殖細(xì)胞核抗原(PCNA)單克隆抗體

Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)

Cyclin

  • 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 產(chǎn)品包裝(模擬)
  • 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 產(chǎn)品包裝(模擬)
  • 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 20μg/ml Mouse Anti-Human PCNA Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 DAB staining on IHC-P;
    Sample: Human Breast cancer Tissue
    Primary Ab: 20μg/ml Mouse Anti-Human PCNA Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 FITC staining on IF;
    Sample: MCF7 cell
    Primary Ab: 20μg/ml Mouse Anti-Human PCNA Antibody
    Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 FITC staining on IF;
    Samples: Human MCF7 cell;
    Primary Ab: 20μg/ml Mouse Anti-Human PCNA Antibody
    Second Ab: 0.75μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu18)
  • MAA591Hu21.jpg Figure. Western Blot; Sample: Recombinant PCNA.
  • 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 Western Blot Sample: Lane1: Porcine Kidney lysate; Lane2: Bovine Testis lysate; Lane3: Caprine Lung lysate; Lane4: Caprine Testis lysate; Lane5: Cavia Lung lysate; Lane6: Cavia Colon lysate; Lane7: Cavia Thymus lysate; Lane8: Equine Liver lysate
    Primary Ab: 1μg/ml Mouse Anti-Human PCNA Antibody
    Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對PCNA的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別PCNA。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

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編號 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA591Hu01 抗增殖細(xì)胞核抗原(PCNA)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CAA591Hu01 抗抗增殖細(xì)胞核抗原(PCNA)多克隆抗體 Loading Control of WB.
PAA591Hu01 抗增殖細(xì)胞核抗原(PCNA)多克隆抗體 WB; IHC; ICC; IP.
MAA591Hu21 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 WB; IHC; ICC; IP.
MAA591Hu22 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 WB; IHC; ICC; IP.
MAA591Hu23 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 WB; IHC; ICC; IP.
MAA591Hu25 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 WB; IHC; ICC; IP.
MAA591Hu24 抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 WB; IHC; ICC; IP.
CAA591Hu22 抗抗增殖細(xì)胞核抗原(PCNA)單克隆抗體 Loading Control of WB.
KSA591Hu01 抗增殖細(xì)胞核抗原(PCNA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
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Food and Chemical Toxicology Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats [Pubmed:30026088]
Life?Sciences Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096]
Archives of Physiology and Biochemistry Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin [Pubmed: 30513216]
European journal of medicinal chemistry Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity [Pubmed: 31216504]
Antioxidants (Basel). The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets [Pubmed: 31426309]
Antioxidants Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian?… [Pubmed: 31600920]
TOXICOLOGY Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis [Pubmed: 32565124]
BIOMEDICINE & PHARMACOTHERAPY Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939]
Sci Rep Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats [33750916]
J Cell Sci Local DNA synthesis is critical for DNA repair during oocyte maturation [34415018]
Stem Cell Rev Rep Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic?… [34859376]
Naunyn-Schmiedeberg's Archives Inflammatory Modulation of miR-155 Inhibits Doxorubicin?Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus?… [Pubmed:35715546]
Antioxidants Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model?… [Pubmed:35204140]
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… []
Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?… []
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