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抗增殖細(xì)胞核抗原(PCNA)單克隆抗體

Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)

Cyclin

編號MAA591Mi23
物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(克隆號)C8
Ig亞型 IgG1 Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPA591Mi01-抗增殖細(xì)胞核抗原(PCNA)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度1mg/mL
且適物種Cavia (Guinea pig ，豚鼠), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Chicken (Gallus，雞)
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
下載英文說明書中文說明書
規(guī)格 20μl100μl 200μl 1ml 10ml
價格 ¥ 770 1798 ¥ 1798 ¥ 2568 ¥ 6420 ¥ 25680
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 20μg/ml Mouse Anti-Multi-species PCNA Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)

Western Blot; Sample: Lane1: Mouse Testis lysate; Lane2: Jurkat cell lysate; Lane3: Rat Testis lysate Primary Ab: 3μg/ml Mouse Anti-Multi-species PCNA Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: A549 cell lysate; Lane2: 293T cell lysate; Lane3: A875 cell lysate; Lane4: HepG2 cell lysate; Lane5: Hela cell lysate; Lane6: YAC1 cell lysate; Lane7: MCF7 cell lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Sp2/0 cell lysate; Lane2: SGC7901 cell lysate; Lane3: ECV304 cell lysate; Lane4: PC3 cell lysate; Lane5: A375 cell lysate; Lane6: A431 cell lysate; Lane7: Jurkat cell lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Mouse Small intestine lysate; Lane2: Mouse Lymph node lysate; Lane3: Mouse Ovary lysate; Lane4: Mouse Thymus lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Porcine Heart lysate; Lane3: Porcine Liver lysate; Lane4: Porcine Spleen lysate; Lane5: Cavia Spleen lysate; Lane6: Caprine Heart lysate; Lane7: Caprine Liver lysate; Lane8: Gallus Cerebrum lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species PCNA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對PCNA的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別PCNA。

用法

Western blottin:g: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時熒光定量PCR實(shí)驗(yàn)服務(wù)

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Tissue and Cell	Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects [Pubmed:27233913]
Food and Chemical Toxicology	Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats [Pubmed:30026088]
Life?Sciences	Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096]
Archives of Physiology and Biochemistry	Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin [Pubmed: 30513216]
European journal of medicinal chemistry	Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity [Pubmed: 31216504]
Antioxidants (Basel).	The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets [Pubmed: 31426309]
Antioxidants	Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian?… [Pubmed: 31600920]
TOXICOLOGY	Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis [Pubmed: 32565124]
BIOMEDICINE & PHARMACOTHERAPY	Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939]
Sci Rep	Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats [33750916]
J Cell Sci	Local DNA synthesis is critical for DNA repair during oocyte maturation [34415018]
Stem Cell Rev Rep	Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic?… [34859376]
Naunyn-Schmiedeberg's Archives	Inflammatory Modulation of miR-155 Inhibits Doxorubicin?Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus?… [Pubmed:35715546]
Antioxidants	Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model?… [Pubmed:35204140]
	Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… []
	Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?… []

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編號	適用物種：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA591Mi01	抗增殖細(xì)胞核抗原(PCNA)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAA591Mi01	抗增殖細(xì)胞核抗原(PCNA)多克隆抗體	WB; IHC; ICC; IP.
MAA591Mi22	抗增殖細(xì)胞核抗原(PCNA)單克隆抗體	WB; IHC; ICC; IP.
MAA591Mi23	抗增殖細(xì)胞核抗原(PCNA)單克隆抗體	WB; IHC; ICC; IP.
CAA591Mi22	抗抗增殖細(xì)胞核抗原(PCNA)單克隆抗體	Loading Control of WB
MAA591Mi24	抗增殖細(xì)胞核抗原(PCNA)單克隆抗體	WB; IHC; ICC; IP.
MAA591Mi21	抗增殖細(xì)胞核抗原(PCNA)單克隆抗體	WB; IHC; ICC; IP.
SEA591Mi	抗增殖細(xì)胞核抗原(PCNA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA591Mi	抗增殖細(xì)胞核抗原(PCNA)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.

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