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腦啡肽酶(CD10)重組蛋白

Recombinant Neprilysin (CD10)

NEP; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase

編號(hào)RPB785Hu02
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來(lái)源原核表達(dá)
宿主E.coli
內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
亞細(xì)胞定位細(xì)胞膜
預(yù)測(cè)分子量22.3kDa
實(shí)際分子量22kDa(差異分析請(qǐng)參閱說(shuō)明書)
片段與標(biāo)簽Phe565~Trp750 with N-terminal His Tag
緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
性狀凍干粉
純度> 97%
等電點(diǎn)6.6
應(yīng)用 Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見(jiàn)活性蛋白。
下載英文說(shuō)明書中文說(shuō)明書
規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

Figure . Gene Sequencing (extract)

SDS-PAGE圖

通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

序列

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

聚丙烯酰胺凝膠電泳(PAGE)實(shí)驗(yàn)服務(wù)

增值服務(wù)

去內(nèi)毒素試劑盒
 BCA蛋白定量檢測(cè)試劑盒
 蛋白標(biāo)記定制服務(wù)
蛋白分子量MARKER
重組蛋白定制服務(wù)
單克隆抗體定制服務(wù)
多克隆抗體定制服務(wù)
蛋白活性測(cè)定實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
緩沖液
 內(nèi)毒素去除試劑盒Ⅱ
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
棘突蛋白受體結(jié)合結(jié)構(gòu)域
 蛋白G
蛋白 A

相關(guān)產(chǎn)品

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
APB785Hu61	腦啡肽酶(CD10)活性蛋白	Cell?culture;?Activity?Assays.
RPB785Hu02	腦啡肽酶(CD10)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPB785Hu01	腦啡肽酶(CD10)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
EPB785Hu61	腦啡肽酶(CD10)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAB785Hu01	腦啡肽酶(CD10)多克隆抗體	WB; IHC; ICC; IP.
PAB785Hu02	腦啡肽酶(CD10)多克隆抗體	WB; IHC; ICC; IP.
LAB785Hu71	腦啡肽酶(CD10)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAB785Hu21	腦啡肽酶(CD10)單克隆抗體	WB; IHC; ICC; IP.
MAB785Hu23	腦啡肽酶(CD10)單克隆抗體	WB; IHC; ICC; IP.
MAB785Hu24	腦啡肽酶(CD10)單克隆抗體	WB; IHC; ICC; IP.
MAB785Hu22	腦啡肽酶(CD10)單克隆抗體	WB; IHC; ICC; IP.
MAB785Hu25	腦啡肽酶(CD10)單克隆抗體	WB; IHC; ICC; IP.
MAB785Hu27	腦啡肽酶(CD10)單克隆抗體	WB; IHC; ICC; IP.
MAB785Hu29	腦啡肽酶(CD10)單克隆抗體	WB; IHC; ICC; IP.
MAB785Hu26	腦啡肽酶(CD10)單克隆抗體	WB; IHC; ICC; IP.
FAB785Hu02	抗腦啡肽酶(CD10)單克隆抗體	FCM
FAB785Hu04	抗腦啡肽酶(CD10)單克隆抗體	FCM
FAB785Hu06	抗腦啡肽酶(CD10)單克隆抗體	FCM
FAB785Hu08	抗腦啡肽酶(CD10)單克隆抗體	FCM
FAB785Hu00	抗腦啡肽酶(CD10)單克隆抗體	Flow cytometry.
FAB785Hu82	抗腦啡肽酶(CD10)單克隆抗體(異硫氰酸熒光素標(biāo)記)	FCM
SEB785Hu	腦啡肽酶(CD10)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB785Hu	腦啡肽酶(CD10)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志	參考文獻(xiàn)
13	Elevated Plasma levels of Neutral Endpeptidase in Hypertension Patients Treated with Angiotensin Converting Enzyme Inhibitors [Ahajournals:Source]
Biol Pharm Bull	Effect of High Glucose Levels on Amyloid β Production in Retinas of Spontaneous Diabetes Mellitus Otsuka Long-Evans Tokushima Fatty Rats [PubMed: 25832640]
Eur J Heart Fail.?	Soluble neprilysin does not correlate with outcome in heart failure with preserved ejection fraction [Pubmed:26725876]
8	A?Test?in?Context:?Neprilysin:?Function,?Inhibition, and?Biomarker. [pubmed:27491909]
European?Journal of?Heart?Failure	Effects of sacubitril/valsartan on neprilysin targets and the metabolism of natriuretic peptides in chronic heart failure: a mechanistic clinical study [Pubmed: 30520545]
European?Journal of?Heart?Failure	Increased granulocyte membrane neprilysin (CD10) expression is associated with better prognosis in heart failure [Pubmed: 30828920]
Clinical Research in Cardiology	Soluble neprilysin, NT-proBNP, and growth differentiation factor-15 as biomarkers for heart failure in dialysis patients (SONGBIRD) [Pubmed: 32002632]
Neurotoxicology	Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice [Pubmed: 32522471]
European Heart Journal	P1620 Association of granulocyte neprilysin (CD10) expression with prognosis in heart failure with reduced ejection fraction []
Clin Biochem	Finding a reliable assay for soluble neprilysin [Pubmed:35331754]
Scientific Reports	The potential role of serum tau protein (MAPT), neuronal cell adhesion molecule (NrCAM) and neprilysin (NEP) in neurodegenerative disorders development in?… []

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