對(duì)氧磷酶1(PON1)重組蛋白
Recombinant Paraoxonase 1 (PON1)
ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1
- 編號(hào)RPA243Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 來源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位分泌
- 預(yù)測(cè)分子量20.8kDa
- 實(shí)際分子量21kDa(差異分析請(qǐng)參閱說明書)
- 片段與標(biāo)簽Gln35~Val206 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)5.1
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請(qǐng)參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1085 ¥ 2712 ¥ 5424 ¥ 16272 ¥ 40680
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序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
贈(zèng)品
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相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA243Hu02 | 對(duì)氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA243Hu01 | 對(duì)氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA243Hu01 | 對(duì)氧磷酶1(PON1)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA243Hu03 | 對(duì)氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA243Hu04 | 對(duì)氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA243Hu05 | 對(duì)氧磷酶1(PON1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA243Hu01 | 對(duì)氧磷酶1(PON1)多克隆抗體 | WB; IHC; ICC; IP. |
PAA243Hu02 | 對(duì)氧磷酶1(PON1)多克隆抗體 | WB; IHC; ICC; IP. |
LAA243Hu71 | 對(duì)氧磷酶1(PON1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
LAA243Hu81 | 對(duì)氧磷酶1(PON1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
MAA243Hu22 | 對(duì)氧磷酶1(PON1)單克隆抗體 | WB; IHC; ICC; IP. |
SEA243Hu | 對(duì)氧磷酶1(PON1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA243Hu | 對(duì)氧磷酶1(PON1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA243Hu | 對(duì)氧磷酶1(PON1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA243Hu01 | 對(duì)氧磷酶1(PON1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Digestive Diseases and Sciences | Fatty Liver Is Associated with Recurrent Bacterial Infections Independent of Metabolic Syndrome [PubMed: 20205513] |
Clinica Chimica Acta | Persistent elevation of paraoxonase-1 specific enzyme activity after weight reduction in obese non-diabetic men with metabolic syndrome [PubMed: 21704607] |
Journal of Laparoendoscopic & Advanced Surgical Techniques | Combined Serum Proteomic and Metabonomic Profiling After Laparoscopic Sleeve Gastrectomy in Children and Adolescents [Wiley: source] |
PLoS One. | High density lipoprotein structural changes and drug response in lipidomic profiles following the long-term fenofibrate therapy in the FIELD substudy [PubMed: 21887280] |
Atherosclerosis | Macrophage cholesterol efflux to plasma and HDL in subjects with low and high homocysteine levels: a FIELD substudy [PubMed: 21696738] |
Lipids in Health and Disease | Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome [PubMed: PMC3480889] |
British Journal of Nutrition | Extra-virgin olive oil consumption reduces the age-related decrease in HDL and paraoxonase 1 anti-inflammatory activities [Pubmed: 23510814] |
Diabetes & Metabolic Syndrome | Paraoxonase 1: A better atherosclerotic risk predictor than HDL in type 2 diabetes mellitus [Pubmed: 23680251] |
Redox Report | Paraoxonase responses to exercise and niacin therapy in men with metabolic syndrome [Pubmed:25180827] |
Toxicol Ind Health. | Influence of α-tocopherol and α-lipoic acid on bisphenol-A-induced oxidative damage in liver and ovarian tissue of rats [Pubmed:25548375] |
RETOS. Nuevas Tendencias en Educación Física, Deporte y Recreación | A single session of aerobic exercise influences paraoxonase 1 activity and concentration [Articulo: 345738764042] |
PLoS One | Plasma Protein Biomarkers of Hepatocellular Carcinoma in HCV-Infected Alcoholic Patients with Cirrhosis [PubMed: 25789864] |
Renal Failure | Association of lipid profile with serum PON1 concentration in patients with chronic kidney disease [Pubmed:26888625] |
Pharmacological reports | The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder [science:article] |
Clinical and Experimental Hypertension | Association of paraoxonase 1 (PON1) gene polymorphisms and concentration with essential hypertension [pubmed:27668323] |
Diabetes & Metabolic Syndrome: Clinical Research & Reviews | Paraoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot Study in North Indian population [pubmed:29254890] |
Diabetes/Metabolism?Research?and Reviews | A Case-control Study: The Association of Serum Paraoxonase 1 Activity and Concentration with the Development of Type 2 Diabetes Mellitus [pubmed:29156090] |
Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
Pharmacological Reports | The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. [pubmed:28623707] |
American Journal of Food Science and Health | Palm Wine and Orijin Bitters Severed Hyperlipidemia and Immunomodulatory Responses in Atherogenic-diet Fed Male Wistar Rats [ISSN: 2381-7224] |
Metabolic brain disease | Individuals with autism have higher 8-Iso-PGF2α levels than controls, but no correlation with quantitative assay of Paraoxonase 1 serum levels [pubmed:28808839] |
Therapeutic Apheresis And Dialysis | Serum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney Disease [Pubmed:29368426] |
Biological?Trace?Element?Research | Effect of Aqueous Extract of?Glycyrrhiza glabra?on the Biochemical Changes Induced by Cadmium Chloride in Rats [Pubmed: 30251228] |
GLYCOCONJUGATE JOURNAL | Identification a novel clinical biomarker in early diagnosis of human non-small cell lung cancer [Pubmed: 30607521] |
Hematology. | Differentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktails [Pubmed: 30661467] |
Proteomics Clinical Applications | Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy [Pubmed: 31162828] |
Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures [Pubmed: 31639605] |
Nutrition, Metabolism and Cardiovascular Diseases | Phenotypes and concentration of PON1 in cardiovascular disease: the role of nutrient intake [Pubmed: 31757567] |
Environment International | Estimating renal and hepatic clearance rates of organophosphate esters in humans: Impacts of intrinsic metabolism and binding affinity with plasma proteins [Pubmed: 31783242] |
RESPIRATORY RESEARCH | The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation?… [Pubmed: 33148285] |
CLINICAL RHEUMATOLOGY | PON-1 haplotype (-108C> T, L55M, and Q192R) modulates the serum levels and activity PONase promoting an atherogenic lipid profile in rheumatoid arthritis patients [Pubmed: 32556934] |
respiratory research | The adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunction [] |
J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555] |
PLoS One | Vitamin D status and systemic redox biomarkers in adults with obesity [30973911] |
Oxid Med Cell Longev | Repetitions of Strenuous Exercise Consistently Increase Paraoxonase 1 Concentration and Activity in Plasma of Average-Trained Men [34917230] |
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