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細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)多克隆抗體

Polyclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2)

MAPK1; P38; P40; ERK2; ERT1; MAPK2; P42MAPK; P42-MAPK; PRKM1; PRKM2; P41; P41mapk; P41-mapk; Mitogen-Activated Protein Kinase 1; Mitogen-activated protein kinase 2

編號PAA930Hu02
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPA930Hu02-細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度0.5mg/mL
且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Chicken (Gallus，雞)
應(yīng)用WB; IHC; ICC; IP; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
價格 ¥ 662 1546 ¥ 1546 ¥ 2208 ¥ 5520 ¥ 22080
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Porcine Small intestine Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ERK2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Porcine Liver Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human ERK2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Skeletal muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 20μg/ml Rabbit Anti-Human IGF1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant ERK2, Human.
Western Blot Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Hela cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Canine Cerebrum lysate; Lane2: Gallus Cerebrum lysate; Lane3: Bovine Cerebrum lysate; Lane4: Cavia Cerebrum lysate; Lane5: Caprine Cerebrum lysate
Primary Ab: 0.05μg/ml Rabbit Anti-Human ERK2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human K562 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human ERK2 Polyclonal Antibody (Catalog PAA930Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對ERK2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別ERK2。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實驗服務(wù)
免疫組織化學(xué)(IHC)實驗服務(wù)
免疫細(xì)胞化學(xué)(ICC)實驗服務(wù)
流式細(xì)胞術(shù)(FCM)實驗服務(wù)
免疫沉淀(IP)實驗服務(wù)
免疫熒光(IF)實驗服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實時熒光定量PCR實驗服務(wù)

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
APA930Hu01	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)活性蛋白	Cell?culture;?Activity?Assays.
RPA930Hu01	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA930Hu02	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAA930Hu01	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)多克隆抗體	WB; IHC; ICC; IP.
PAA930Hu02	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)多克隆抗體	WB; IHC; ICC; IP; FCM
CAA930Hu01	抗細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)多克隆抗體	Loading Control of WB
MAA930Hu23	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	WB; IHC; ICC; IP.
MAA930Hu22	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	WB; IHC
MAA930Hu27	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	WB; IHC; ICC; IP.
MAA930Hu26	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	WB; IHC; ICC; IP.
MAA930Hu28	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	WB; IHC; ICC; IP.
MAA930Hu24	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	WB; IHC; ICC; IP.
CAA930Hu22	抗細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	Loading Control of WB
MAA930Hu25	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	WB; IHC; ICC; IP.
MAA930Hu21	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	WB; IHC; ICC; IP.
FAA930Hu04	抗細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	Flow cytometry.
FAA930Hu06	抗細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	Flow cytometry.
FAA930Hu02	抗細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	Flow cytometry.
FAA930Hu08	抗細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)單克隆抗體	Flow cytometry.
MEA930Hu	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)	Enzyme-linked immunosorbent assay for Antigen Detection.
SEA930Hu	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA930Hu	細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Cellular and Molecular Neurobiology	Hypericum Perforatum?Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease [Pubmed:26119304]
Graefe's Archive for Clinical and Experimental Ophthalmology	Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome [pubmed:27538909]
International Journal of Reproductive Medicine	Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis [33575317]

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