己糖激酶2(HK2)多克隆抗體
Polyclonal Antibody to Hexokinase 2 (HK2)
HK II; Muscle form hexokinase
- 編號PAD352Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPD352Hu02-己糖激酶2(HK2)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.39mg/mL
- 且適物種Mus musculus (Mouse，小鼠)
- 應用WB; IHC; ICC; IP; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 10μg/ml Rabbit Anti-Human HK2 Antibody Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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Figure: FITC staining on IHC-P;
Sample: MCF7 cells.
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WB圖
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Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 5μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: HK2 knockout MCF7 cell lysate;
Predicted MW: 102kd
Observed MW: 102kd
Primary Ab: 5μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 5μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human A431 cell lysate;
Primary Ab: 5μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 5μg/ml Rabbit Anti-Human HK2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human HK2 Polyclonal Antibody (Catalog PAD352Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對HK2的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別HK2。
用法
Western blotting: 0.01-5μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPD352Hu01 | 己糖激酶2(HK2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD352Hu02 | 己糖激酶2(HK2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD352Hu01 | 己糖激酶2(HK2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAD352Hu02 | 己糖激酶2(HK2)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| MAD352Hu21 | 己糖激酶2(HK2)單克隆抗體 | WB; IHC; ICC; IP. |
| SED352Hu | 己糖激酶2(HK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD352Hu | 己糖激酶2(HK2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Anti-Cancer Drugs | 3-Bromopyruvate enhances TRAIL-induced apoptosis in human nasopharyngeal carcinoma cells through CHOP-dependent upregulation of TRAIL-R2. [10.1097/CAD.0000000000000502] |
| Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC) [] | |
| J Cell Mol Med | Huaier shows anti?\cancer activities by inhibition of cell growth, migration and energy metabolism in lung cancer through PI3K/AKT/HIF?\1|á pathway [33377619] |
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