Kelch樣ECH關(guān)聯(lián)蛋白1(KEAP1)多克隆抗體
Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1)
INrf2; KLHL19; Cytosolic inhibitor of Nrf2; Kelch-like protein 19
- 編號PAL648Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPL648Hu01-Kelch樣ECH關(guān)聯(lián)蛋白1(KEAP1)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 691 ¥ 1613 ¥ 2304 ¥ 5760 ¥ 23040
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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IHC圖
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FITC staining on IF;
Samples: Human HepG2 Cells;
Primary Ab: 20μg/ml Rabbit Anti-Human KEAP1 Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant KEAP1, Human.
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Western Blot; Samples: Lane1: 293T cell lysate; Lane2: HepG2 cell lysate; Lane3: Rat Placenta lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human KEAP1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: KEAP1 knockout 293T cell lysate;
Predicted MW: 70kd
Observed MW: 65kd
Primary Ab: 1μg/ml Rabbit Anti-Human KEAP1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Mouse Lung lysate; Lane2: Mouse Cerebrum lysate; Lane3: Mouse Placenta lysate; Lane4: Mouse Kidney lysate; Lane5: NIH/3T3 cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human KEAP1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對KEAP1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別KEAP1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPL648Hu01 | Kelch樣ECH關(guān)聯(lián)蛋白1(KEAP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAL648Hu01 | Kelch樣ECH關(guān)聯(lián)蛋白1(KEAP1)多克隆抗體 | WB; IHC; ICC/IF |
| MAL648Hu21 | Kelch樣ECH關(guān)聯(lián)蛋白1(KEAP1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEL648Hu | Kelch樣ECH關(guān)聯(lián)蛋白1(KEAP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LML648Hu | Kelch樣ECH關(guān)聯(lián)蛋白1(KEAP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Environmental Toxicology and Pharmacology | Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study [pubmed:28192751] |
| Journal of?Infection?and Chemotherapy | Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicity [Pubmed:29753615] |
| Immunopharmacology?and?immunotoxicology | Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosis [Pubmed: 30488751] |
| International Immunopharmacology | S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammation [Pubmed: 30665040] |
| Respiratory physiology & neurobiology | Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis [Pubmed: 31600583] |
| Vet Sci | Protective and Therapeutic Effects of Orlistat on Metabolic Syndrome and Oxidative Stress in High-Fat Diet-Induced Metabolic Dysfunction-Associated Fatty Liver?… [34822647] |
| Antioxidants (Basel) | Hepatoprotective Effect of Bee Bread in Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Rats: Impact on Oxidative Stress and Inflammation [34943134] |
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