甘露糖結合凝集素1(LMAN1)多克隆抗體
Polyclonal Antibody to Lectin, Mannose Binding 1 (LMAN1)
F5F8D; FMFD1; MCFD1; MR60; Gp58; Protein ERGIC-53; Coagulation Factor V-Factor VIII Combined Deficiency; Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 53
- 編號PAC567Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPC567Hu01-甘露糖結合凝集素1(LMAN1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種-
- 應用WB,IHC,ICC/IF; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human LMAN1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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FITC staining on IF;<br/>Sample: Human Hela cell; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human LMAN1 Antibody<br/>Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant LMAN1, Human.
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Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human LMAN1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: LMAN1 knockout Hela cell lysate;
Predicted MW: 58kd
Observed MW: 58kd
Primary Ab: 1μg/ml Rabbit Anti-Human LMAN1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human LMAN1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human LMAN1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human LMAN1 Polyclonal Antibody (Catalog PAC567Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對LMAN1的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別LMAN1。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunofluorescence:5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPC567Hu01 | 甘露糖結合凝集素1(LMAN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC567Hu01 | 甘露糖結合凝集素1(LMAN1)多克隆抗體 | WB,IHC,ICC/IF; FCM |
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