微管蛋白β(TUBb)重組蛋白
Recombinant Tubulin Beta (TUBb)
TUB-B; TUBB5; TUBB1; M40
- 編號RPB870Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位細胞質(zhì)
- 預(yù)測分子量32.1kDa
- 實際分子量32kDa(差異分析請參閱說明書)
- 片段與標簽Val170~Val419 with N-terminal His Tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點6.6
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1104 ¥ 2760 ¥ 5520 ¥ 16560 ¥ 41400
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序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APB870Hu01 | 微管蛋白β(TUBb)活性蛋白 | Cell?culture;?Activity?Assays. |
RPB870Hu01 | 微管蛋白β(TUBb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CAB870Hu01 | 抗微管蛋白β(TUBb)多克隆抗體 | Loading Control of WB. |
PAB870Hu01 | 微管蛋白β(TUBb)多克隆抗體 | WB; IHC; ICC/IF |
MAB870Hu22 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
MAB870Hu23 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
MAB870Hu25 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
MAB870Hu27 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
MAB870Hu24 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
MAB870Hu21 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
CAB870Hu22 | 抗微管蛋白β(TUBb)單克隆抗體 | Loading Control of WB. |
FAB870Hu06 | 抗微管蛋白β(TUBb)單克隆抗體 | Flow cytometry. |
FAB870Hu04 | 抗微管蛋白β(TUBb)單克隆抗體 | Flow cytometry. |
FAB870Hu02 | 抗微管蛋白β(TUBb)單克隆抗體 | Flow cytometry. |
FAB870Hu08 | 抗微管蛋白β(TUBb)單克隆抗體 | Flow cytometry. |
參考文獻
雜志 | 參考文獻 |
archives of pharmacal research | Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues [pubmed:27747473] |
archiv der pharmazie (Weinheim). | New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition. [pubmed:28150327] |
ACS?Applied?Materials?&?Interfaces | Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixture [pubmed:28112513] |
Future?Medicinal Chemistry | Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agents [Pubmed:29779400] |
Future?Medicinal Chemistry | Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerization [Pubmed:29966433] |
Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167] |
Journal?of?Enzyme?Inhibition?and?Medicinal?Chemistry | Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2Inhibitors [Pubmed: 30191744] |
European Journal of Medicinal Chemistry | Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking study [Pubmed: 31536891] |
ACS Sensors | Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platform [Pubmed: 32207606] |
Bioorganic Chemistry | Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their?… [Pubmed: 32325332] |
BRITISH JOURNAL OF NUTRITION | Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats [Pubmed: 33028437] |
European Journal of Medicinal Chemistry | Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon?… [Pubmed: 33158579] |
J BIOMOL STRUCT DYN/JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | Cytotoxicity, in silico predictions and molecular studies for androstane heterocycle compounds revealed potential antitumor agent against lung cancer cells [33300466] |
Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins [] |
Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212] |
Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
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