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甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(化學發(fā)光免疫分析法)

CLIA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH

  • 甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(化學發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(化學發(fā)光免疫分析法) 產(chǎn)品包裝(模擬)
  • 甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(化學發(fā)光免疫分析法) 實驗結(jié)果圖
  • SCB932Hu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測甘油醛-3-磷酸脫氫酶(GAPDH)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。

實驗原理

將甘油醛-3-磷酸脫氫酶(GAPDH)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的甘油醛-3-磷酸脫氫酶(GAPDH)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的甘油醛-3-磷酸脫氫酶(GAPDH)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的甘油醛-3-磷酸脫氫酶(GAPDH)呈正相關(guān)。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。

相關(guān)產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應用(僅供研究使用，不用于臨床診斷！)
RPB932Hu02 甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB932Hu03 甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB932Hu01 甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB932Hu01 甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體 WB; IHC; ICC; IP.
CAB932Hu01 抗甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體 Loading Control of WB.
PAB932Hu02 甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體 WB; IHC; ICC; IP; FCM
LAB932Hu71 甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體(生物素標記) WB; IHC; ICC.
MAB932Hu24 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 WB; IHC; ICC; IP.
MAB932Hu25 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 WB; IHC; ICC; IP.
MAB932Hu27 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 WB; IHC; ICC; IP.
MAB932Hu29 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 WB; IHC; ICC; IP.
MAB932Hu22 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 WB; IHC; ICC; IP.
MAB932Hu26 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 WB; IHC; ICC; IP.
MAB932Hu28 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 WB; IHC; ICC; IP.
CAB932Hu22 抗甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 Loading Control of WB;IHC;IF
MAB932Hu21 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 WB; IHC; ICC; IP.
MAB932Hu23 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 WB; IHC; ICC; IP.
SEB932Hu 甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB932Hu 甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(化學發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB932Hu 甘油醛-3-磷酸脫氫酶(GAPDH)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

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