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甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體

Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH

編號MAB932Hu25
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(克隆號)F1
Ig亞型 IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPB932Hu01-甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/mL
且適物種-
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。
下載英文說明書中文說明書
規(guī)格 20μl100μl 200μl 1ml 10ml
價格 ¥ 657 1533 ¥ 1533 ¥ 2190 ¥ 5475 ¥ 21900
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Human Breast cancer Tissue Primary Ab: 10μg/ml Mouse Anti-Human GAPDH Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 10μg/ml Mouse Anti-Human GAPDH Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Western Blot; Samples: Lane1: Porcine Liver lysate; Lane2: Porcine Kidney lysate; Lane3: Gallus Kidney lysate; Lane4: Rabbit Liver lysate; Lane5: Rabbit Kidney lysate; Lane6: Mouse Liver lysate;
Primary Ab: 0.5μg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對GAPDH的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別GAPDH。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。

贈品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實驗服務(wù)
免疫組織化學(xué)(IHC)實驗服務(wù)
免疫細(xì)胞化學(xué)(ICC)實驗服務(wù)
流式細(xì)胞術(shù)(FCM)實驗服務(wù)
免疫沉淀(IP)實驗服務(wù)
免疫熒光(IF)實驗服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實時熒光定量PCR實驗服務(wù)

相關(guān)產(chǎn)品

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB932Hu02	甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPB932Hu03	甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPB932Hu01	甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAB932Hu01	甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體	WB; IHC; ICC; IP.
CAB932Hu01	抗甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體	Loading Control of WB.
PAB932Hu02	甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體	WB; IHC; ICC; IP; FCM
LAB932Hu71	甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAB932Hu24	甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體	WB; IHC; ICC; IP.
MAB932Hu25	甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體	WB; IHC; ICC; IP.
MAB932Hu27	甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體	WB; IHC; ICC; IP.
MAB932Hu29	甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體	WB; IHC; ICC; IP.
MAB932Hu22	甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體	WB; IHC; ICC; IP.
MAB932Hu26	甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體	WB; IHC; ICC; IP.
MAB932Hu28	甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體	WB; IHC; ICC; IP.
CAB932Hu22	抗甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體	Loading Control of WB;IHC;IF
MAB932Hu21	甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體	WB; IHC; ICC; IP.
MAB932Hu23	甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體	WB; IHC; ICC; IP.
SEB932Hu	甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
SCB932Hu	甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(化學(xué)發(fā)光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
LMB932Hu	甘油醛-3-磷酸脫氫酶(GAPDH)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Clin Cancer Res	Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402]
Clin Cancer Res	Inactivation of the p53–KLF10–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402]
Diabetes & Metabolism	Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells. [pubmed:28499695]
Scientific Reports	Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egression [pubmed:28720868]
Journal of Biological Chemistry	Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167]
Biomaterials	Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels [Pubmed: 30343256]
Bulletin of Experimental Biology and Medicine	Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells [Pubmed: 30488202]
Journal?of?Medicinal?Chemistry	Discovery, Optimization and Target Identification of Novel Potent Broad-Spectrum Antiviral Inhibitors [Pubmed: 30938999]
International Immunopharmacology	Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway [Pubmed: 31078923]
Cancers	Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia [Pubmed: 31718075]
Biological research	Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … [Pubmed: 32046779]
Biomed Pharmacother	Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway [Pubmed: 32028241]
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Cancers (Basel)	BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 581 [33540760]
MOLECULAR CANCER RESEARCH	Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity [33262291]
Sci Rep	Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026]
Cancer Rep (Hoboken)	Curcumin alters distinct molecular pathways in breast cancer subtypes revealed by integrated miRNA/mRNA expression analysis [Pubmed:34981672]
Prog Neurobiol	Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegeneration [Pubmed:35151792]
Plant Molecular Biology	Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiency [Pubmed:35577991]

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