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血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Angiotensin I Converting Enzyme (ACE)

CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

  • 血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) 實(shí)驗(yàn)結(jié)果圖
  • SEA004Mu.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測血管緊張素轉(zhuǎn)化酶(ACE)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的血管緊張素轉(zhuǎn)化酶(ACE)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 96-104 99
heparin plasma(n=5) 98-105 101

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的血管緊張素轉(zhuǎn)化酶(ACE)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管緊張素轉(zhuǎn)化酶(ACE)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 84-99% 81-103% 90-99% 87-104%
EDTA plasma(n=5) 89-101% 78-102% 81-96% 79-102%
heparin plasma(n=5) 82-105% 79-89% 80-102% 78-99%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將血管緊張素轉(zhuǎn)化酶(ACE)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的血管緊張素轉(zhuǎn)化酶(ACE)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血管緊張素轉(zhuǎn)化酶(ACE)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的血管緊張素轉(zhuǎn)化酶(ACE)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA004Mu03 血管緊張素轉(zhuǎn)化酶(ACE)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA004Mu01 血管緊張素轉(zhuǎn)化酶(ACE)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA004Mu02 血管緊張素轉(zhuǎn)化酶(ACE)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA004Mu03 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體 WB; IHC; ICC; IP.
PAA004Mu01 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體 WB; IHC; ICC; IP.
PAA004Mu02 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體 WB; IHC; ICC; IP.
LAA004Mu71 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
SEA004Mu 血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA004Mu 血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA004Mu 血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
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Вавиловский журнал генетики и селекции Ренин-ангиотензин-альдостероновая система у крыс линии НИСАГ (ISIAH) со стресс-индуцированной артериальной гипертензией [30_Dubinina.pdf]
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Biomedicine & Pharmacotherapy Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro- L -arginine methyl ester [pubmed:28992471]
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Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
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Biotechnology and Pharmacy Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats []
Dikkat Eksikli?i Hiperaktivite Bozuklu?u Tan?s? Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin D?nü?türücü Enzim (ACE), NADPH Oksidaz Enzim Serum?… []
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