細(xì)胞外信號(hào)調(diào)節(jié)激酶1(ERK1)重組蛋白
Recombinant Extracellular Signal Regulated Kinase 1 (ERK1)
HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; MAPK3; ERT2; Mitogen-Activated Protein Kinase 3; Insulin-stimulated MAP2 kinase; Microtubule-associated protein 2 kinase
- 編號(hào)RPB357Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 來(lái)源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位細(xì)胞核, 細(xì)胞質(zhì)
- 預(yù)測(cè)分子量31.3kDa
- 實(shí)際分子量28kDa(差異分析請(qǐng)參閱說(shuō)明書(shū))
- 片段與標(biāo)簽Pro75~Thr312 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)8.7
-
應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請(qǐng)參見(jiàn)活性蛋白。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
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相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB357Hu01 | 細(xì)胞外信號(hào)調(diào)節(jié)激酶1(ERK1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB357Hu01 | 細(xì)胞外信號(hào)調(diào)節(jié)激酶1(ERK1)多克隆抗體 | WB; IHC; ICC; IP. |
CAB357Hu01 | 抗細(xì)胞外信號(hào)調(diào)節(jié)激酶1(ERK1)多克隆抗體 | Loading Control of WB. |
MAB357Hu22 | 細(xì)胞外信號(hào)調(diào)節(jié)激酶1(ERK1)單克隆抗體 | WB; IHC; ICC; IP. |
MAB357Hu21 | 細(xì)胞外信號(hào)調(diào)節(jié)激酶1(ERK1)單克隆抗體 | WB; IHC; ICC; IP. |
CAB357Hu22 | 抗細(xì)胞外信號(hào)調(diào)節(jié)激酶1(ERK1)單克隆抗體 | Loading Control of WB. |
SEB357Hu | 細(xì)胞外信號(hào)調(diào)節(jié)激酶1(ERK1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB357Hu | 細(xì)胞外信號(hào)調(diào)節(jié)激酶1(ERK1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Graefe's Archive for Clinical and Experimental Ophthalmology | Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome [pubmed:27538909] |
International Journal of Reproductive Medicine | Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis [33575317] |
Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
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