細胞外信號調(diào)節(jié)激酶1(ERK1)單克隆抗體
Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)
HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; MAPK3; ERT2; Mitogen-Activated Protein Kinase 3; Insulin-stimulated MAP2 kinase; Microtubule-associated protein 2 kinase
- 編號MAB357Hu21
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C2
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB357Hu01-細胞外信號調(diào)節(jié)激酶1(ERK1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 778 ¥ 1814 ¥ 2592 ¥ 6480 ¥ 25920
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Colon Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對ERK1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別ERK1。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB357Hu01 | 細胞外信號調(diào)節(jié)激酶1(ERK1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB357Hu01 | 細胞外信號調(diào)節(jié)激酶1(ERK1)多克隆抗體 | WB; IHC; ICC; IP. |
| CAB357Hu01 | 抗細胞外信號調(diào)節(jié)激酶1(ERK1)多克隆抗體 | Loading Control of WB. |
| MAB357Hu22 | 細胞外信號調(diào)節(jié)激酶1(ERK1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB357Hu21 | 細胞外信號調(diào)節(jié)激酶1(ERK1)單克隆抗體 | WB; IHC; ICC; IP. |
| CAB357Hu22 | 抗細胞外信號調(diào)節(jié)激酶1(ERK1)單克隆抗體 | Loading Control of WB. |
| SEB357Hu | 細胞外信號調(diào)節(jié)激酶1(ERK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB357Hu | 細胞外信號調(diào)節(jié)激酶1(ERK1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome [pubmed:27538909] |
| International Journal of Reproductive Medicine | Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis [33575317] |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
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