角蛋白18(CK18)單克隆抗體
Monoclonal Antibody to Cytokeratin 18 (CK18)
KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18
- 編號MAB231Hu23
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)F5
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPB231Hu01-角蛋白18(CK18)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應用WB; ICC/IF
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 655 ¥ 1529 ¥ 2184 ¥ 5460 ¥ 21840
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 20μg/ml Mouse Anti-Human KRT18 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
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FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 30ug/ml Mouse Anti-Human CK18 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
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Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Rat Liver lysate; Lane3: Rat Kidney lysate Primary Ab: 2μg/ml Mouse Anti-Human KRT18 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human CK18 Monoclonal Antibody (Catalog MAB231Hu23, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對CK18的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別CK18。
用法
Western blotting: 0.01-2μg/mL;
Immunocytochemistry: 5-30μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB231Hu01 | 角蛋白18(CK18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB231Hu02 | 角蛋白18(CK18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB231Hu03 | 角蛋白18(CK18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB231Hu03 | 角蛋白18(CK18)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| PAB231Hu01 | 角蛋白18(CK18)多克隆抗體 | WB; IHC; ICC/IF |
| LAB231Hu81 | 角蛋白18(CK18)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB231Hu71 | 角蛋白18(CK18)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAB231Hu27 | 角蛋白18(CK18)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB231Hu24 | 角蛋白18(CK18)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB231Hu21 | 角蛋白18(CK18)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB231Hu22 | 角蛋白18(CK18)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB231Hu23 | 角蛋白18(CK18)單克隆抗體 | WB; ICC/IF |
| MAB231Hu25 | 角蛋白18(CK18)單克隆抗體 | WB; IHC; ICC; IP. |
| FAB231Hu02 | 抗角蛋白18(CK18)單克隆抗體 | Flow cytometry. |
| FAB231Hu08 | 抗角蛋白18(CK18)單克隆抗體 | Flow cytometry. |
| FAB231Hu06 | 抗角蛋白18(CK18)單克隆抗體 | Flow cytometry. |
| FAB231Hu04 | 抗角蛋白18(CK18)單克隆抗體 | Flow cytometry. |
| SEB231Hu | 角蛋白18(CK18)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB231Hu | 角蛋白18(CK18)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB231Hu01 | 角蛋白18(CK18)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Can J Physiol Pharmacol.? | Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis [Pubmed:27186801] |
| J Alzheimers Dis | Cigarette Smoke-Induced Alterations in Frontal White Matter Lipid Profiles Demonstrated by MALDI-Imaging Mass Spectrometry: Relevance to Alzheimer's Disease [Pubmed:26836183] |
| bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring [] |
| Biomed Chromatogr | Lipidomic‐based investigation into the therapeutic effects of PPC and BBD on rats with NAFLD [34727379] |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver?… [Pubmed:35212074] |
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