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角蛋白18(CK18)單克隆抗體

Monoclonal Antibody to Cytokeratin 18 (CK18)

KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18

編號(hào)MAB231Hu21
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源單抗制備
宿主小鼠
效價(jià)(克隆號(hào))E4
Ig亞型 IgG1 Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPB231Hu01-角蛋白18(CK18)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度1mg/mL
且適物種-
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 10μg/ml Mouse Anti-Human KRT18 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human KRT18 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Endometrial cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human KRT18 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human KRT18 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human KRT18 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;<br/>Samples: Human HepG2 cell; <br/>Primary Ab: 20μg/ml Mouse Anti-Human KRT18 Antibody<br/>Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu18)

Figure. Western Blot; Sample: Recombinant KRT18, Human.
Western Blot; Sample: Rat Liver lysate
Primary Ab: 3μg/ml Mouse Anti-Human KRT18 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
WB圖
WB圖
Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human CK18 Monoclonal Antibody (Catalog MAB231Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)CK18的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CK18。

用法

Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB231Hu01	角蛋白18(CK18)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPB231Hu02	角蛋白18(CK18)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPB231Hu03	角蛋白18(CK18)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAB231Hu03	角蛋白18(CK18)多克隆抗體	WB; IHC; ICC; IP; FCM
PAB231Hu01	角蛋白18(CK18)多克隆抗體	WB; IHC; ICC/IF
LAB231Hu81	角蛋白18(CK18)多克隆抗體(異硫氰酸熒光素標(biāo)記)	WB; IHC; ICC; IF.
LAB231Hu71	角蛋白18(CK18)多克隆抗體(生物素標(biāo)記)	WB; IHC; ICC.
MAB231Hu27	角蛋白18(CK18)單克隆抗體	WB; IHC; ICC; IP.
MAB231Hu24	角蛋白18(CK18)單克隆抗體	WB; IHC; ICC; IP.
MAB231Hu21	角蛋白18(CK18)單克隆抗體	WB; IHC; ICC; IP.
MAB231Hu22	角蛋白18(CK18)單克隆抗體	WB; IHC; ICC; IP.
MAB231Hu23	角蛋白18(CK18)單克隆抗體	WB; ICC/IF
MAB231Hu25	角蛋白18(CK18)單克隆抗體	WB; IHC; ICC; IP.
FAB231Hu02	抗角蛋白18(CK18)單克隆抗體	Flow cytometry.
FAB231Hu08	抗角蛋白18(CK18)單克隆抗體	Flow cytometry.
FAB231Hu06	抗角蛋白18(CK18)單克隆抗體	Flow cytometry.
FAB231Hu04	抗角蛋白18(CK18)單克隆抗體	Flow cytometry.
SEB231Hu	角蛋白18(CK18)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB231Hu	角蛋白18(CK18)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSB231Hu01	角蛋白18(CK18)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Can J Physiol Pharmacol.?	Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis [Pubmed:27186801]
J Alzheimers Dis	Cigarette Smoke-Induced Alterations in Frontal White Matter Lipid Profiles Demonstrated by MALDI-Imaging Mass Spectrometry: Relevance to Alzheimer's Disease [Pubmed:26836183]
bioscience biotechnology research asia	Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring []
Biomed Chromatogr	Lipidomic‐based investigation into the therapeutic effects of PPC and BBD on rats with NAFLD [34727379]
J Magn Reson Imaging	Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver?… [Pubmed:35212074]

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