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表皮生長因子受體(EGFR)多克隆抗體

Polyclonal Antibody to Epidermal Growth Factor Receptor (EGFR)

ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

編號(hào)PAA757Hu02
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 所有
- 人
- 小鼠
- 大鼠
- 豚鼠
- 家兔
- 獼猴
- 山羊
- 綿羊
- 馬
- 牛
- 豬
- 雞
- 狗
- 其他物種
- 多物種
- 泛物種
來源多抗制備
宿主兔
效價(jià)-
Ig類型 IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原 RPA757Hu02-表皮生長因子受體(EGFR)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度0.5mg/mL
且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
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規(guī)格 20μl100μl 200μl 1ml 10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)

DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human EGFR Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human EGFR Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human EGFR Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 20ug/ml Rabbit Anti-Human EGFR Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20ug/ml Rabbit Anti-Human EGFR Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Western Blot; Sample: Lane1: A431 cell lysate; Lane2: Human Placenta lysate; Lane3: Porcine Cerebrum lysate Primary Ab: 0.1μg/ml Rabbit Anti-Human EGFR Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Human MCF7(3ul) cell lysate; <br/>Primary Ab: 1μg/ml Rabbit Anti-Human EGFR Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human EGFR Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)EGFR的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別EGFR。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

增值服務(wù)

抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
 細(xì)胞和組織染色液
 抗體陽性對(duì)照品
 組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
 DAB顯色試劑盒
 SABC試劑盒
 長臂生物素標(biāo)記試劑盒
 實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)

相關(guān)產(chǎn)品

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
APA757Hu01	表皮生長因子受體(EGFR)活性蛋白	Cell?culture;?Activity?Assays.
APA757Hu02	表皮生長因子受體(EGFR)活性蛋白	Cell?culture;?Activity?Assays.
APA757Hu62	表皮生長因子受體(EGFR)活性蛋白	Cell?culture;?Activity?Assays.
RPA757Hu01	表皮生長因子受體(EGFR)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APA757Hu61	表皮生長因子受體(EGFR)活性蛋白	Cell?culture;?Activity?Assays.
EPA757Hu62	表皮生長因子受體(EGFR)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA757Hu02	表皮生長因子受體(EGFR)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
EPA757Hu61	表皮生長因子受體(EGFR)真核蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAA757Hu06	表皮生長因子受體(EGFR)多克隆抗體	WB; IHC; ICC; IP.
PAA757Hu02	表皮生長因子受體(EGFR)多克隆抗體	WB; IHC; ICC; IP.
PAA757Hu01	表皮生長因子受體(EGFR)多克隆抗體	WB; IHC; ICC; IP.
FAA757Hu51	抗表皮生長因子受體(EGFR)多克隆抗體(別藻藍(lán)蛋白標(biāo)記)	FCM
MAA757Hu27	表皮生長因子受體(EGFR)單克隆抗體	WB; IHC; ICC; IP.
MAA757Hu29	表皮生長因子受體(EGFR)單克隆抗體	WB; IHC; ICC; IP.
RAA757Hu21	表皮生長因子受體(EGFR)重組抗體	WB; IF; ICC; IHC; IP; FCM.
MAA757Hu21	表皮生長因子受體(EGFR)單克隆抗體	WB; IHC; ICC; IP.
MAA757Hu23	表皮生長因子受體(EGFR)單克隆抗體	WB; IHC; ICC; IP.
MAA757Hu24	表皮生長因子受體(EGFR)單克隆抗體	WB; IHC; ICC; IP.
MAA757Hu25	表皮生長因子受體(EGFR)單克隆抗體	WB; IHC; ICC; IP.
MAA757Hu22	表皮生長因子受體(EGFR)單克隆抗體	WB; IHC
SEA757Hu	表皮生長因子受體(EGFR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
HEA757Hu	表皮生長因子受體(EGFR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型)	Enzyme-linked immunosorbent assay for Antigen Detection.
AEA757Hu	抗表皮生長因子受體抗體(Anti-EGFR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antibody Detection.
LMA757Hu	表皮生長因子受體(EGFR)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志	參考文獻(xiàn)
Thoracic Cancer	Nimotuzumab combined with gemcitabine and cisplatin as second-line chemotherapy for advanced non-small-cell lung cancer [Wiley: source]
J Cent Nerv Syst Dis.?	Increased Epidermal Growth Factor Receptor (EGFR) Associated with Hepatocyte Growth Factor (HGF) and Symptom Severity in Children with Autism … [Pubmed:25249767]
Mol Cancer Ther	GC1118, an anti-EGFR antibody with a distinct binding epitope and superior inhibitory activity against high-affinity EGFR ligands [PubMed: 26586721]
Cell Death Dis.?	LINC01225 promotes occurrence and metastasis of hepatocellular carcinoma in an epidermal growth factor receptor-dependent pathway [Pubmed:26938303]
analytica chimica acta	Microfluidic immunosensor based on mesoporous silica platform and CMK-3/poly-acrylamide-co-methacrylate of dihydrolipoic acid modified gold electrode for cancer biomarker detection. [pubmed:28335979]
Talanta	EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform [Doi: 10.1016/j.talanta.2018.10.016]
Bioorganic Chemistry	Design, synthesis, molecular docking and biological activity evaluation of some novel indole derivatives as potent anticancer active agents and apoptosis inducers [Pubmed: 30665034]
Journal of?enzyme?inhibition and medicinal chemistry	Novel thienopyrimidine derivatives as dual EGFR and VEGFR-2 inhibitors: design, synthesis, anticancer activity and effect on cell cycle profile [Pubmed: 30919701]
Bioorganic Chemistry	Design and synthesis of new benzoxazole/benzothiazole-phthalimide hybrids as antitumor-apoptotic agents []
Bioorganic Chemistry	Synthesis and anticancer activity of bis-benzo [d][1, 3] dioxol-5-yl thiourea derivatives with molecular docking study [Pubmed: 31288134]
Oxidative Medicine and Cellular Longevity	Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters?… [Pubmed: 31737175]
Neoplasia	Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364]
	Association between Serum Epidermal Growth Factor Receptor and Cyclooxygenase-2 Levels in Patients with Non-small Cell Carcinoma of Lung []
	Synthesis, EGFR-TK inhibition and anticancer activity of new quinoxaline derivatives []
IMMUNOLOGIC RESEARCH	Humoral immune response to epidermal growth factor receptor in lung cancer [33495907]
European Journal of Medicinal Chemistry	Pyrazolo[3,4-d]pyrimidine-based dual EGFR T790M/HER2 inhibitors: Design, synthesis, structure¨Cactivity relationship and biological activity as potential antitumor and anticonvulsant agents [33545637]
Journal of Molecular Structure	Design, synthesis, antiproliferative evaluation, and molecular docking study of new quinoxaline derivatives as apoptotic inducers and EGFR inhibitors []
ACS Appl Mater Interfaces	Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions [34914377]
Bioorganic Chemistry	Discovery of new pyrimido [5, 4-c] quinolines as potential antiproliferative agents with multitarget actions: Rapid synthesis, docking, and ADME studies [Pubmed:35219045]

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