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中期因子(MK)多克隆抗體

Polyclonal Antibody to Midkine (MK)

MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2

  • 中期因子(MK)多克隆抗體 產(chǎn)品包裝(模擬)
  • 中期因子(MK)多克隆抗體 產(chǎn)品包裝(模擬)
  • 中期因子(MK)多克隆抗體 DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human MK Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 中期因子(MK)多克隆抗體 DAB staining on IHC-P;
    Samples: Human Stomach cancer Tissue;
    Primary Ab: 10μg/ml Rabbit Anti-Human MK Antibody
    Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 中期因子(MK)多克隆抗體 DAB staining on IHC-P;
    Sample: Human Stomach cancer Tissue
    Primary Ab: 10μg/ml Rabbit Anti-Human MK Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 中期因子(MK)多克隆抗體 AF488 staining on IF;
    Sample: Hela cell
    Primary Ab: 20μg/ml Rabbit Anti-Human MK Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA631Hu02.jpg WB圖
  • 中期因子(MK)多克隆抗體 Western Blot; Sample: Lane1: Rat Placenta lysate; Lane2: Porcine Cerebrum lysate
    Primary Ab: 2μg/ml Rabbit Anti-Human MK Antibody
    Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 中期因子(MK)多克隆抗體 Western Blot; Sample: SH-SY5Y cell lysate
    Primary Ab: 0.5μg/ml Rabbit Anti-Human MK Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

該抗體是針對(duì)MK的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別MK。

用法

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.

儲(chǔ)存

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

贈(zèng)品

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編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA631Hu02 中期因子(MK)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA631Hu01 中期因子(MK)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA631Hu01 中期因子(MK)活性蛋白 Cell?culture;?Activity?Assays.
PAA631Hu02 中期因子(MK)多克隆抗體 WB; IHC; ICC; IP.
PAA631Hu01 中期因子(MK)多克隆抗體 WB; IHC; ICC; IP.
LAA631Hu71 中期因子(MK)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
LAA631Hu81 中期因子(MK)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
MAA631Hu22 中期因子(MK)單克隆抗體 WB; IHC; ICC; IP.
MAA631Hu21 中期因子(MK)單克隆抗體 WB; IHC; ICC; IP.
SEA631Hu 中期因子(MK)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA631Hu 中期因子(MK)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA631Hu01 中期因子(MK)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Midkine: From Embryogenesis to Pathogenesis and Therapy Immunochemical Methods for the Detection of Midkine in Samples of Human Origin [Springer: Source]
33 Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … [10.1016:j.ebiom.2017.11.020]
Circulation Journal Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice [pubmed:28781288]
EBioMedicine Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and?… [Pubmed:29233575]
Journal of Clinical Laboratory Analysis Clinical value of jointly detection pleural fluid Midkine, pleural fluid adenosine deaminase, and pleural fluid carbohydrate antigen 125 in the identification of nonsmall?… [Pubmed:29797475]
Charakterisierung der funktionalen Rolle des Zytokins Midkine bei der Arteriogenese [:]
Scientific Reports The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440]
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337]
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