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胰島素降解酶(IDE)多克隆抗體

DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human IDE Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human IDE Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IDE Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Testis Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IDE Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IDE Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IDE Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant IDE, Human.
Figure. Western Blot; Sample: Human HepG2 Cells.
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: IDE knockout HepG2 cell lysate;
Predicted MW: 118,54kd
Observed MW: 100kd
Primary Ab: 1μg/ml Rabbit Anti-Human IDE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Cerebrum lysate
Primary Ab: 0.01μg/ml Rabbit Anti-Human IDE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: K562 cell lysate
Primary Ab: 0.4μg/ml Rabbit Anti-Human IDE Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Cerebrum lysate
Primary Ab: 0.01μg/ml Rabbit Anti-Human IDE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

該抗體是針對(duì)IDE的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別IDE。

Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.

經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

雜志	參考文獻(xiàn)
J Neurochem	Reduction [PubMed: 26442993]
African Journal of Pharmacy and Pharmacology	Peptide hydroxamate derivatives as regulators for insulin receptor signaling and its degradation by zinc metalloprotease in diabetic rats [article-full-text-pdf]
Biomedicine & Pharmacotherapy	Vitamin?D3?intake?as?regulator?of?insulin?degrading?enzyme?and?insulin?receptor?phosphorylationin?diabetic?rats. [pubmed:27930980]
Biological Trace Element Research	Nigella sativa Oil and Chromium Picolinate Ameliorate Fructose-Induced Hyperinsulinemia by Enhancing Insulin Signaling and Suppressing Insulin-Degrading Enzyme in Male Rats [10.1007/s12011-017-1167-z]
Neuroscience	The Protective Effects of IGF-I against β-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase A [Pubmed:29406271]

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編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB897Hu01	胰島素降解酶(IDE)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
APB897Hu01	胰島素降解酶(IDE)活性蛋白	Cell?culture;?Activity?Assays.
PAB897Hu01	胰島素降解酶(IDE)多克隆抗體	WB; IHC
MAB897Hu21	胰島素降解酶(IDE)單克隆抗體	WB; IHC; ICC; IP.
MAB897Hu22	胰島素降解酶(IDE)單克隆抗體	WB; IHC; ICC; IP.
SEB897Hu	胰島素降解酶(IDE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB897Hu	胰島素降解酶(IDE)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSB897Hu01	胰島素降解酶(IDE)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	自己動(dòng)手制作ELISA試劑盒的主要材料