胰島素降解酶(IDE)重組蛋白
Recombinant Insulin Degrading Enzyme (IDE)
Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase
- 編號RPB897Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位細胞核, 分泌, 細胞質(zhì)
- 預測分子量29.7kDa
- 實際分子量30kDa(差異分析請參閱說明書)
- 片段與標簽Ala753~Pro973 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 90%
- 等電點7.2
-
應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1166 ¥ 2916 ¥ 5832 ¥ 17496 ¥ 43740
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序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。
增值服務
相關產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPB897Hu01 | 胰島素降解酶(IDE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB897Hu01 | 胰島素降解酶(IDE)活性蛋白 | Cell?culture;?Activity?Assays. |
PAB897Hu01 | 胰島素降解酶(IDE)多克隆抗體 | WB; IHC |
MAB897Hu21 | 胰島素降解酶(IDE)單克隆抗體 | WB; IHC; ICC; IP. |
MAB897Hu22 | 胰島素降解酶(IDE)單克隆抗體 | WB; IHC; ICC; IP. |
SEB897Hu | 胰島素降解酶(IDE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB897Hu | 胰島素降解酶(IDE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB897Hu01 | 胰島素降解酶(IDE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
J Neurochem | Reduction [PubMed: 26442993] |
African Journal of Pharmacy and Pharmacology | Peptide hydroxamate derivatives as regulators for insulin receptor signaling and its degradation by zinc metalloprotease in diabetic rats [article-full-text-pdf] |
Biomedicine & Pharmacotherapy | Vitamin?D3?intake?as?regulator?of?insulin?degrading?enzyme?and?insulin?receptor?phosphorylationin?diabetic?rats. [pubmed:27930980] |
Biological Trace Element Research | Nigella sativa Oil and Chromium Picolinate Ameliorate Fructose-Induced Hyperinsulinemia by Enhancing Insulin Signaling and Suppressing Insulin-Degrading Enzyme in Male Rats [10.1007/s12011-017-1167-z] |
Neuroscience | The Protective Effects of IGF-I against β-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase A [Pubmed:29406271] |
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